EOS 7D Mark II擁有EOS史上最多的65個自動對焦點，且全部為十字型※。其對焦點高密度地覆蓋了畫面大部分空間。拍攝時，在優先確定好構圖的情況下，也能在所需的位置直接對焦；就算拍攝在畫面內激烈運動的被攝體，也可以針對目標位置迅速對焦。

※搭配的鏡頭不同，自動對焦點、十字型自動對焦點以及雙十字型自動對焦點的可用數量也會發生變化。

65點自動對焦系統可用的自動對焦點數量、類型及分布因使用鏡頭而有所不同，共分為A至G七組，可從下表查看所使用的鏡頭所屬組別。使用E至G組鏡頭時的可用自動對焦點較少。

f/2.8 雙十字型自動對焦點 f/5.6 十字型自動對焦點 f/5.6 垂直自動對焦點 (對水平線條敏感) f/5.6 水平自動對焦點 (對垂線條敏感)

A 組 65個十字型自動對焦點 (包括1個雙十字型自動對焦點) EF 14mm f/2.8L II USM EF 50mm f/1.8 EF 400mm f/2.8L II USM EF 14mm f/2.8L USM EF 85mm f/1.2L II USM EF 400mm f/2.8L USM EF 15mm f/2.8 Fisheye EF 85mm f/1.2L USM TS-E45mm f/2.8* EF 20mm f/2.8 USM EF 85mm f/1.8 USM TS-E90mm f/2.8* EF 24mm f/1.4L II USM EF 100mm f/2 USM EF 16-35mm f/2.8L II USM EF 24mm f/1.4L USM EF 135mm f/2.8 (Softfocus) EF 16-35mm f/2.8L USM EF 24mm f/2.8 IS USM EF 135mm f/2L USM EF 17-35mm f/2.8L USM EF 24mm f/2.8 EF 135mm f/2L USM + Extender EF 1.4x EF 20-35mm f/2.8L EF 28mm f/1.8 USM EF 200mm f/1.8L USM EF 24-70mm f/2.8L II USM EF 28mm f/2.8 IS USM EF 200mm f/1.8L USM + Extender EF 1.4x^ EF 24-70mm f/2.8L USM EF 28mm f/2.8 EF 200mm f/2.8L II USM EF 28-70mm f/2.8L USM EF 35mm f/1.4L USM EF 200mm f/2.8L USM EF 70-200mm f/2.8L IS II USM EF 35mm f/2 IS USM EF 200mm f/2L IS USM EF 70-200mm f/2.8L IS USM EF 35mm f/2 EF 200mm f/2L IS USM + Extender EF 1.4x EF 70-200mm f/2.8L USM EF 40mm f/2.8 STM EF 300mm f/2.8L IS II USM EF 80-200mm f/2.8L EF 50mm f/1.0L USM EF 300mm f/2.8L IS USM EF-S 24mm f/2.8 STM EF 50mm f/1.2L USM EF 300mm f/2.8L USM EF-S 17-55mm f/2.8 IS USM EF 50mm f/1.4 USM EF 400mm f/2.8L IS II USM EF 50mm f/1.8 II EF 400mm f/2.8L IS USM B 組 65個十字型自動對焦點 EF 50mm f/2.5 Compact Macro EF 500mm f/4L IS II USM + Extender EF 1.4x EF 70-200mm f/2.8L IS II USM + Extender EF 2x EF 50mm f/2.5 Compact Macro + LIFE SIZE Converter EF 500mm f/4L IS USM EF 70-200mm f/2.8L IS USM + Extender EF 1.4x EF 100mm f/2.8 Macro EF 500mm f/4L IS USM + Extender EF 1.4x EF 70-200mm f/2.8L IS USM + Extender EF 2x EF 100mm f/2.8L Macro IS USM EF 500mm f/4.5L USM EF 70-200mm f/2.8L USM + Extender EF 1.4x^ EF 135mm f/2L USM + Extender EF 2x EF 600mm f/4L IS II USM EF 70-200mm f/2.8L USM + Extender EF 2x^ EF 180mm f/3.5L Macro USM EF 600mm f/4L IS II USM + Extender EF 1.4x EF 70-200mm f/4L IS USM EF 200mm f/1.8L USM + Extender EF 2x^ EF 600mm f/4L IS USM EF 70-200mm f/4L IS USM + Extender EF 1.4x EF 200mm f/2.8L II USM + Extender EF 1.4x EF 600mm f/4L IS USM + Extender EF 1.4x EF 70-200mm f/4L USM EF 200mm f/2.8L II USM + Extender EF 2x EF 600mm f/4L USM EF 70-200mm f/4L USM + Extender EF 1.4x EF 200mm f/2.8L USM + Extender EF 1.4x EF 600mm f/4L USM + Extender EF 1.4x^ EF 70-210mm f/3.5-4.5 USM EF 200mm f/2.8L USM + Extender EF 2x TS-E 17mm f/4L* EF 70-210mm f/4 EF 200mm f/2L IS USM + Extender EF 2x TS-E 24mm f/3.5L* EF 70-300mm f/4-5.6 IS USM EF 300mm f/2.8L IS II USM + Extender EF 1.4x TS-E 24mm f/3.5L II* EF 70-300mm f/4-5.6L IS USM EF 300mm f/2.8L IS II USM + Extender EF 2x EF 8-15mm f/4L Fisheye USM EF 70-300mm f/4.5-5.6 DO IS USM EF 300mm f/2.8L IS USM + Extender EF 1.4x EF 16-35mm f/4L IS USM EF 75-300mm f/4-5.6 IS USM EF 300mm f/2.8L IS USM + Extender EF 2x EF 17-40mm f/4L USM EF 75-300mm f/4-5.6 III USM EF 300mm f/2.8L USM + Extender EF 1.4x^ EF 24-70mm f/4L IS USM EF 75-300mm f/4-5.6 III EF 300mm f/2.8L USM + Extender EF 2x^ EF 24-105mm f/3.5-5.6 IS STM EF 75-300mm f/4-5.6 II USM EF 300mm f/4L IS USM EF 24-105mm f/4L IS USM EF 75-300mm f/4-5.6 II EF 300mm f/4L IS USM + Extender EF 1.4x EF 28-80mm f/2.8-4L USM EF 75-300mm f/4-5.6 EF 300mm f/4L USM EF 28-90mm f/4-5.6 II USM EF 100-200mm f/4.5A EF 300mm f/4L USM + Extender EF 1.4x EF 28-90mm f/4-5.6 USM EF 100-300mm f/5.6L EF 400mm f/2.8L IS II USM + Extender EF 1.4x EF 28-90mm f/4-5.6 III EF 100-300mm f/5.6 EF 400mm f/2.8L IS II USM + Extender EF 2x EF 28-90mm f/4-5.6 II EF 100-400mm f/4.5-5.6L IS II USM EF 400mm f/2.8L IS USM + Extender EF 1.4x EF 28-90mm f/4-5.6 EF 100-400mm f/4.5-5.6L IS USM EF 400mm f/2.8L IS USM + Extender EF 2x EF 28-105mm f/3.5-4.5 II USM EF 200-400mm f/4L IS USM Extender 1.4x EF 400mm f/2.8L II USM + Extender EF 1.4x^ EF 28-105mm f/3.5-4.5 USM EF 200-400mm f/4L IS USM Extender 1.4x + Extender EF 1.4x EF 400mm f/2.8L II USM + Extender EF 2x^ EF 28-135mm f/3.5-5.6 IS USM EF 200-400mm f/4L IS USM Extender 1.4x: 內置增距鏡設定為1.4x EF 400mm f/2.8L USM + Extender EF 1.4x^ EF 28-200mm f/3.5-5.6 EF-S 60mm f/2.8 Macro USM EF 400mm f/2.8L USM + Extender EF 2x^ EF 28-200mm f/3.5-5.6 USM EF-S 15-85mm f/3.5-5.6 IS USM EF 400mm f/4 DO IS II USM EF 28-300mm f/3.5-5.6L IS USM EF-S 17-85mm f/4-5.6 IS USM EF 400mm f/4 DO IS II USM + Extender EF 1.4x EF 35-105mm f/3.5-4.5 EF-S 18-135mm f/3.5-5.6 IS EF 400mm f/4 DO IS USM EF 35-135mm f/3.5-4.5 EF-S 18-135mm f/3.5-5.6 IS STM EF 400mm f/4 DO IS USM + Extender EF 1.4x EF 50-200mm f/3.5-4.5L EF-S 18-200mm f/3.5-5.6 IS EF 400mm f/5.6L USM EF 50-200mm f/3.5-4.5 EF-S 55-250mm f/4-5.6 IS EF 500mm f/4L IS II USM EF 70-200mm f/2.8L IS II USM + Extender EF 1.4x EF-S 55-250mm f/4-5.6 IS II 　 EF-S 55-250mm f/4-5.6 IS STM C 組 45個十字型自動對焦點 20個 垂直自動對焦點 EF 20-35mm f/3.5-4.5 USM EF-S 18-55mm f/3.5-5.6 IS STM EF-S 18-55mm f/3.5-5.6 III EF 35-135mm f/4-5.6 USM EF-S 18-55mm f/3.5-5.6 IS II EF-S 18-55mm f/3.5-5.6 II EF 75-300mm f/4-5.6 USM EF-S 18-55mm f/3.5-5.6 IS EF-S 18-55mm f/3.5-5.6 EF 100-300mm f/4.5-5.6 USM EF-S 18-55mm f/3.5-5.6 II USM EF-S 10-22mm f/3.5-4.5 USM EF-S 18-55mm f/3.5-5.6 USM D 組 25個十字型自動對焦點 40個 垂直自動對焦點 EF 24-85mm f/3.5-4.5 USM EF 80-200mm f/4.5-5.6 EF 35-350mm f/3.5-5.6L USM EF 90-300mm f/4.5-5.6 USM EF 55-200mm f/4.5-5.6 II USM EF 90-300mm f/4.5-5.6 EF 55-200mm f/4.5-5.6 USM E 組 25個十字自動對焦點 20個垂直自動對焦點 EF 100mm f/2.8 Macro USM EF 28-80mm f/3.5-5.6 III USM EF 35-80mm f/4-5.6 PZ EF 800mm f/5.6L IS USM EF 28-80mm f/3.5-5.6 II USM EF 35-80mm f/4-5.6 II EF 1200mm f/5.6L USM EF 28-80mm f/3.5-5.6 USM EF 38-76mm f/4.5-5.6 EF 28-70mm f/3.5-4.5 II EF 28-80mm f/3.5-5.6 II EF 80-200mm f/4.5-5.6 USM EF 28-70mm f/3.5-4.5 EF 28-80mm f/3.5-5.6 EF 80-200mm f/4.5-5.6 II EF 28-80mm f/3.5-5.6 V USM EF 35-70mm f/3.5-4.5A EF-S 10-18mm f/4.5-5.6 IS STM EF 28-80mm f/3.5-5.6 IV USM EF 35-70mm f/3.5-4.5 F 組 15個十字自動對焦點 20個垂直自動對焦點 10個水平自動對焦點 EF 180mm f/3.5L Macro USM + Extender EF 1.4x EF 35-80mm f/4-5.6 USM EF 22-55mm f/4-5.6 USM EF 35-80mm f/4-5.6 III EF 28-105mm f/4-5.6 USM EF 35-80mm f/4-5.6 EF 28-105mm f/4-5.6 G 組(f/5.6) 1個十字自動對焦點 2個垂直自動對焦點 2個水平自動對焦點 EF 35-105mm f/4.5-5.6 USM EF 35-105mm f/4.5-5.6 G 組(f/8) 1個十字自動對焦點 2個垂直自動對焦點 2個水平自動對焦點 EF 300mm f/4L IS USM + Extender EF 2x EF 500mm f/4L IS USM + Extender EF 2x EF 70-200mm f/4L USM + Extender EF 2x EF 300mm f/4L USM + Extender EF 2x EF 600mm f/4L IS II USM + Extender EF 2x EF 100-400mm f/4.5-5.6L IS II USM + Extender EF 1.4x EF 400mm f/4 DO IS II USM + Extender EF 2x EF 600mm f/4L IS USM + Extender EF 2x EF 100-400mm f/4.5-5.6L IS USM + Extender EF 1.4x EF 400mm f/4 DO IS USM + Extender EF 2x EF 600mm f/4L USM + Extender EF 2x^ EF 200-400mm f/4L IS USM Extender 1.4x + Extender EF 2x EF 400mm f/5.6L USM + + Extender EF 1.4x EF 800mm f/5.6L IS USM + Extender EF 1.4x EF 200-400mm f/4L IS USM Extender 1.4x: 內置增距鏡設定為1.4x + Extender EF 1.4x EF 500mm f/4.5L USM + Extender EF 1.4x^ EF 1200mm f/5.6L USM + Extender EF 1.4x^ 　 EF 500mm f/4L IS II USM + Extender EF 2x EF 70-200mm f/4L IS USM + Extender EF 2x 　 f/8 十字自動對焦點 f/8 垂直自動對焦點 (不能手動選擇) f/8 水平自動對焦點 (不能手動選擇) ^此組合使用自動對焦時可能無法實現精確對焦。 *無傾斜/偏移的手動對焦。